Dendritic Cell Immunization Route
نویسندگان
چکیده
منابع مشابه
Efficiency of Dendritic Cell Vaccination against B16 Melanoma Depends on the Immunization Route
Dendritic cells (DC) presenting tumor antigens are crucial to induce potent T cell-mediated anti-tumor immune responses. Therefore DC-based cancer vaccines have been established for therapy, however clinical outcomes are often poor and need improvement. Using a mouse model of B16 melanoma, we found that the route of preventive DC vaccination critically determined tumor control. While repeated D...
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Exogenous dendritic cells (bone marrow-derived dendritic cell (BMDC)) display restricted trafficking in vivo after injection into mice, but the route(s) by which they generate gut-homing effector cells is unclear. Mesenteric lymph nodes (LN) and spleen were differentially targeted by i.p. and i.v. administration of BMDC, respectively, whereas mediastinal LN were targeted by both routes. BMDC in...
متن کاملDendritic cell vaccines Paul
1. Abstract 2. Introduction 3. Preparation of dendritic cells 3.1. Sources of dendritic cells 3.2. Dendritic cell mobilization strategies 3.3. Dendritic cell culture conditions 3.4. Methods of loading dendritic cells with tumor antigens 3.5. Dendritic cell maturation 3.6. The role of helper antigens 3.7. cryopreservation of dendritic cells 4. Administration of dendritic cell vaccines 4.1. Route...
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We developed a technique for direct inguinal lymph node injection in mice to compare various routes of immunization with tumor lysate-pulsed dendritic cell (DC) vaccines. Syngeneic, bone marrow-derived, tumor lysate-pulsed DCs administered intranodally generated more potent protective antitumor immunity than s.c. or i.v. DC immunizations. Intranodal immunization with ovalbumin peptide-pulsed DC...
متن کاملRoute of Immunization with Peptide-pulsed Dendritic Cells Controls the Distribution of Memory and Effector T Cells in Lymphoid Tissues and Determines the Pattern of Regional Tumor Control
We have established that the route of immunization with peptide-pulsed, activated DC leads to memory CD8+ T cells with distinct distributions in lymphoid tissues, which determines the ability to control tumors growing in different body sites. Both intravenous (i.v.) and subcutaneous (s.c.) immunization induced memory T cells in spleen and control of metastatic-like lung tumors. s.c. immunizatio...
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